Dear Yuri, If you have located all the heavy atoms (sulfur and phosphor) correctly, you could try sulfur-SAD phasing using the program OASIS. This program has a record of solving a 1206 residues protein with SAD signals from 22 sulfur atoms scattered under Cu-Ka radiation and a record of solving a 213 residues protein with SAD signals from 2 sulfur atoms scattered under Cr-Ka radiation.
By the way please note that the OASIS in CCP4 6.2.x is not the uptodate version. You can get a better version from http://cryst.iphy.ac.cn. The latest version will be available on the website in the coming October. Best regards, Hai-fu On Sat, Sep 24, 2011 at 2:49 AM, Yuri Pompeu <yuri.pom...@ufl.edu> wrote: > Hello everyone, > I have a data set >99% completeness to 1.15A > This is a 400 amino acid long protein and it has 7 Met (Sulfur peaks around > 20sigma) > And a tightly bound phosphate (P peak around 22sigma) > Could I try and solve this directly or is it crazy idea? > If so what program should I try? > > thanks > Yuri > >
