Hello everyone, I am refining a structure to 2.4A with 2-fold NCS and twinned. Maps look ok and Rfree is 0.27however as I start checking my validations I notice that after refinemnt my geomtry gets significantly worse. especially the rama plot. Initially I have 2 outliers and I end up with 32 (5%)!!! I played with the Xray weight term but alll it helped me with was rmsd bond/angles, rama is still messing up... Can I impose some ramachadran restraints or maybe have a reference model?
best,
