i wouldn't recoommend that. here is the info from somebody forwarded
from our genetics department with regards to safety of that while
back....
"Sybr is just as toxic/poisonous/harmful as ethidium bromide, only
far more expensive. There have been very few tests concerning it's
use up until now and therefore it should be treated with even more
caution than ethidium bromide. Waste must be separated and processed
by the company and use of Sybr in the lab must involve safety and
care levels at least as stringent as with Ethidium bromide."
(not sure thow if it was SYBR-"safe"... safe must mean safe, right...
in particular with chemical companies...)
Feel free to handle your gels as sloppy as you like (or eat them or
whatever), but mind the other people's health in the lab. There are
people who actually have to work with this stuff for decades. I would
advice following the safety instructions.
Tommi
On Oct 2, 2011, at 9:10 PM, Jacob Keller wrote:
There exists a less toxic chemical than EtBr to stain DNA: SYBR safe
DNA stain (a fluorescence dye sold by a certain vendor).
SYBR Safe is about 10X less sensitive though.
Can you do the toothbrush test with SYBR Safe?
JPK
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
Tommi Kajander, Ph.D.
Junior Group Leader
Structural Biology and Biophysics
Institute of Biotechnology
University of Helsinki
Viikinkaari 1
(P.O. Box 65)
00014 Helsinki
Finland
p. +358-9-19158903
tommi.kajan...@helsinki.fi
