Hi Frank I believe (at least part of) the key to the 30S ribosome structure was being able to orient the crystals carefully and collect the data accordingly - see Brodersen et al, Acta Cryst. (2003). D59, 2044-2050
Completeness, while important, ain't everything. On 18 Nov 2011, at 08:26, Frank von Delft wrote: > I believe you achieve completeness more quickly (fewer crystals) if you just > take random orientations. At least, that's what I learnt from Dave Stuart. > phx > > > > On 18/11/2011 04:20, Frank Murphy wrote: >> Yanwu, >> >> I surmise from your question that you are inquiring how to go about >> collecting from many crystals optimally. Merging data ex post facto is a >> totally different kettle of fish. >> >> In my opinion, the most robust way to go about this is to use a kappa >> goniometer as Jim suggested (I am most familiar with the MK3). Since you >> intend to collect from many crystals, align the first and all subsequent >> crystals to the same easily attainable (or seemingly so) orientation, and >> then collect the sweep suggested by your data collection strategy program of >> choice. >> >> To achieve this at NE-CAT, we have a GUI-based system that used STAC for >> orientation determination and BEST for strategy generation. As Jim >> suggested, more options than STAC exist. >> >> If anyone is unable to get to a kappa goniometer, they can employ Mosflm or >> XDS (Xplan) to generate strategies for data collection from a crystal taking >> into account previously collected data. This is not nearly as robust a >> solution, but is a workable substitute (and also automated at NE-CAT). >> >> I know there are other ways to achieve similar results, but I have suggested >> the methods I am most familiar with... >> >> >> Yours, >> Frank Murphy >> >> >> Begin forwarded message: >> >>> From: yanwu huo <applehu...@gmail.com> >>> Date: November 17, 2011 4:00:06 PM CST >>> To: CCP4BB@JISCMAIL.AC.UK >>> Subject: [ccp4bb] crystal orientation during data collection >>> Reply-To: yanwu huo <applehu...@gmail.com> >>> >>> Hi, >>> I worked on a crystal sensitive to radiation damage, So I need to merge >>> many crystal to obtain complete dataset, Does anyone know such program that >>> can tell crystal orientation after first frame exposure. >>> Thank you in advance. >>> >>> >>> -- >>> Thank you very much and all the best, >>> >>> Yanwu Huo >>> Postdoctoral Associate >>> Department of Molecular Biology and Genetics >>> Cornell University >>> Ithaca, NY, 14853 >>> Email:yh...@cornell.edu >>> >> >> >> >> Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH
