my DNA contains a 9-1-9 palindromic sequence,i tried different blunt end DNA,and got two kind of crystal with different lenght DNA.both don't diffract.
2011/11/21 Michael Murphy <pn1...@gmail.com> > Deng, could you tell us a bit more about the DNA that you used? I think > you may want to try to optimize the DNA that you are using. Maybe try > either a couple base pair longer/shorter piece of DNA? or try switching > from blunt ended DNA to a one or two base pair overhang piece of DNA? (very > often it is contacts between the ends of the pieces of DNA that drive > crystallization) > > On Mon, Nov 21, 2011 at 2:36 AM, dengzq1987 <dengzq1...@gmail.com> wrote: > >> ** >> Hi all, >> >> I got a protein-DNA complex crystal,by running SDS-PAGE and nativePAGE,i >> prove that the crystal contain both >> protein and DNA.BUT it does not diffract. does anyone have suggestion to >> improve the diffraction ability ? >> >> >> Best wishes, >> >> deng >> > >
