Hmm- I wonder if B-factor unsharpening (applying a large POSITIVE B-factor to the data) would have the same effect?
Francis E Reyes wrote:
On the other hand, shooting a lower resolution crystal may get you the conformation of the disordered domain. Surprising at first thought, but was true in p97/VCP from the Brunger Lab. F On Dec 7, 2011, at 11:47 PM, atul kumar wrote:Dear all I have crytals which diffract up to 3.2 A at synchrotron, I am solving this by molecular replacement. I have built 70% of the model successfully,but the problem is it have a very poor density for some 30-40 residues at N terminal. I can't build anything in this region,this could be because of disordered structure or because of low resolution.what are the things which I can try to improve this? suggestion are requested! thanks regards' Atul Kumar
