Generally no, only in the subsequent protein purification steps. Tony.
Sent from my iPhone On 12 Dec 2011, at 21:57, "Yibin Lin" <yyb...@gmail.com> wrote: > Hi List, > > I want to preprare sel-met labeling protein. Could someone can tell me > if it is necessary to add DTT or BME to medium, which contains > sel-met, when growing cells? > > Thanks in advance, > > > Yibin Lin
