hi pretty ,
Once when u will fit the atom in green density (+ve Fo-Fc
map) (Green density means there is some thing missing and you need to
model) You need to refine the structure by using refinement program. while
refining the structure program will calculate Fc and then again it will
generate map by calculating Fo-Fc if your atom is properly fitted Fo-Fc
will go down and green density will disappear.
So first refine the structure and then again see the density for those
atoms.
regards
On Wed, Jan 4, 2012 at 10:51 AM, Dialing Pretty <hdc123hdc...@yahoo.com>wrote:
> Dear All,
>
> Attached is a Screenshot of coot. The blue is the 2FoFcwt map, the green
> is the FoFcwt map.
>
> After I select the FoFcwt map, I have tried to change the green blob to an
> atom. First I point a green blob, then I use
> calculate-Model/Fit/Refine-Pointer Atom Type to add any atom.
>
> My question is, although the atom can be added, there is no indication
> that the volume of the green blob changes. If I am right, the green blob
> should disappear if the atom is correctly selected.
>
> How do you solve this problem? How about the red blobs in the attached
> Screenshot?
>
> I am looking forward to getting your reply.
>
> Cheers,
>
> Dialing
>
--
Vandana kukshal
