I second Stephen's comments on NanoITC. I haven't seen any improvement in terms of volume. The total volume one should use is very much comparable to MicroCal VP-ITC. Analysis software is OK--not so good--crashing some times--GUI needs to bit robust in handling the data.
HTH ! Krishna Chinthalapudi Hannover Medical School On Tue, Jan 17, 2012 at 6:55 PM, Stephen Graham <sc...@cam.ac.uk> wrote: > We bought a NanoITC from TA instruments last year and I can't > recommend it enough. We are able to get excellent binding curves with > the low volume cell/syringe (200uL/50uL, respectively, which actually > means 350uL/120uL in order to load both without bubbles) using the > same concentrations of protein as we used to use in a MicroCal VP-ITC. > The control/analysis software is much easier to use (although the > graphing capabilities could be improved) and the technical support has > thus far has been excellent. Also, as others have said it's a steal > compared with the equivalent GE machine (both in terms of purchase > cost and cost of maintenance contracts). > > Stephen > > On 17 January 2012 16:02, Jose Artur Brito <jbr...@itqb.unl.pt> wrote: > > Dear All, > > sorry for this off-topic questions but I would like to have some > feed-back > > from you on Isothermal Titration Calorimetry (ITC) equipments. > > We have a very nice quotation for an iTC200 from GE Healthcare. We wanted > > this one because it uses ~200uL sample per measurement (really nice when > > your dealing with "precious" samples, ie., proteins with low expression > > yields). However, I was told that, although consuming much less sample, > is > > not as good (sensitivity, mixing issues, bubbles, ...), as the VP-ITC (it > > uses ~1.4mL per measurement, seven times more than the iTC200). > > Does anyone has experience with these two equipments? Would you prefer > one > > over the other (please state your reasons)? Would you suggest another > > equipment/brand for the ITC (like the NanoITC from TA Instruments)? > > Thanks in advance, > > Jose Artur Brito > > > > -- > > ************************************************ > > * José Artur Brito, PhD * > > * * > > * Post-Doctoral Fellow * > > * Membrane Protein Crystallography Lab * > > * Instituto de Tecnologia Química e Biológica * > > * Oeiras - Portugal * > > * * > > * Tel.: +351.21.446.97.61 * > > * Fax: +351.21.443.36.44 * > > * * > > * E-mail: jbr...@itqb.unl.pt * > > * URL: http://mx.itqb.unl.pt * > > ************************************************ > > > > -- > Dr Stephen Graham > 1851 Research Fellow > Cambridge Institute for Medical Research > Wellcome Trust/MRC Building > Addenbrooke's Hospital, Hills Road > Cambridge, CB2 0XY, UK > Phone: +44 1223 762 638 >
