9000 rpm translates to 13,000 g on this centrifuge/rotor. I am not referring to pelleting bacterial cells. My question is about centrifuging bacterial lysates and some recommendations for sturdy tubes.
Thanks. Raji On Tue, Jan 31, 2012 at 12:02 PM, Bosch, Juergen <jubo...@jhsph.edu> wrote: > To how many g does your 9000 rpm translate ? Perhaps that's the problem ? > 10 minutes @ 5000xg for pelleting cells is more than enough in my opinion. > > Jürgen > > On Jan 31, 2012, at 11:59 AM, Raji Edayathumangalam wrote: > > Hi Folks, > > Are you any favorite brands out there for crack-resistant 50mL > centrifugation tubes. It seems we are having recurring episodes of Falcon > and Corning tubes cracking even at 9,000 rpm, which is the maximum speed > possible with our rotor. I have used Falcon tubes for years in the past > without problems and I want to be able to spin down bacterial lysates > without a mess. > > Any suggestions for tubes that have worked well in your experience? > > Thanks, > Raji > > -- > Raji Edayathumangalam > Instructor in Neurology, Harvard Medical School > Research Associate, Brigham and Women's Hospital > Visiting Research Scholar, Brandeis University > > > > ...................... > Jürgen Bosch > Johns Hopkins University > Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns Hopkins Malaria Research Institute > 615 North Wolfe Street, W8708 > Baltimore, MD 21205 > Office: +1-410-614-4742 > Lab: +1-410-614-4894 > Fax: +1-410-955-2926 > http://web.mac.com/bosch_lab/ > > > > > -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
