Hello Everyone, Many many thanks to all the folks who responded to my question with very good suggestions.
Here's a very quick and dirty summary of the various tubes and rotors that people use without any issues: (1) 50ml Nalgene tubes for an SS-34 rotor (2) Shape-matched new Fiberlite rotors (3) Nalgene round-bottom centrifuge tubes (re-usable) (4) Beckman ultracentrifuge tubes (re-usable) (5) 50 ml Falcon tubes (red cap) (6) 50 ml Corning tubes in F13S-14x50cy rotor (7) Polyethylene tubes work but polycarbonate do not, for some folks It seems Fiberlite rotors were a common suggestion and a bunch of folks suggested that breakage may have AS MUCH to do with centrifuge and shape-complementarity (understandably) as much as with the centrifuge tubes. Many thanks for your time and help. Go CCP4BB! Raji ---------- Forwarded message ---------- From: Raji Edayathumangalam <r...@brandeis.edu> Date: Tue, Jan 31, 2012 at 11:59 AM Subject: Crack-resistant tubes for centrifugation To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@jiscmail.ac.uk> Hi Folks, Are you any favorite brands out there for crack-resistant 50mL centrifugation tubes. It seems we are having recurring episodes of Falcon and Corning tubes cracking even at 9,000 rpm, which is the maximum speed possible with our rotor. I have used Falcon tubes for years in the past without problems and I want to be able to spin down bacterial lysates without a mess. Any suggestions for tubes that have worked well in your experience? Thanks, Raji -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
