hi sudhir, i think in second snapshot you can fit MPD as its structure is seems similar. .....
On Fri, Feb 10, 2012 at 11:36 AM, Sudhir Kumar <sudhir.1...@gmail.com>wrote: > Dear all, > I have a 2 A structure of an enzyme which show 12 molecules per > asymmetric unit. While placing waters i found out some blobs where i could > not model any ligand. It is surrounded by 3 Asp molecules. I have attached > screenshot of the blob at 1.5 sigma cutoff 2fo-fc density. Crystallization > condition had following precipitants: PEG 1000, Peg 3350, MPD, Ethylene > glycol,and protein contained Glycerol. My protein is known to bind > Cysteine/Serine, though i haven't added any in crystallization buffer. any > suggestion what it might be are welcome. thanks in advance > > -- > best regards > Sudhir Kumar > Research Scholar > C/O Dr. S. Gourinath > Structural Biology Laboratory > SLS, JNU, > New Delhi-110067 > -- Vandana kukshal
