El 16/02/12 05:06, Xun Lu escribió: > Hi Lisa, > > Please go check your PDB file. Are those bases written out like "DT" or > "THY" or "Td". Coot recognizes certain format for DNA bases but I forgot > which one coot likes. I don't have my laptop with me right now. My > guess would be "Td". :) > > Best, > > Xun > >
Hi, And that is compounded with the fact that depending on your installation, Coot may be using its own libraries or the CCP4 ones. And they may differ, especially if you're using the "new dictionnaries" for refmac5. Plus the fact that depending on your preferences Coot converts atoms to PDB v.2.x so they may not come back as you gave them to it. A mess... Paul, at the very least, it would be helpful if like in Lisa's case, an ideal DNA/RNA is created consistent with whatever libraries Coot is going to use for real space refining it. Best regards, -- Miguel > On Wednesday, February 15, 2012, LISA <science...@gmail.com > <mailto:science...@gmail.com>> wrote: >> Hi all, >> >> I am refining a structue of protein-DNA complex with coot. I add DNA > by "adding ideal DNA/RNA" in the other model. But I cannot edit chi > angle of these nucletide, neither the mutate. When I press the mutate > and my DNA, coot give amino acid not nucletide. Why? >> >> Thanks >> >> Lisa >> > > -- > Department of Molecular and Structural Biochemistry > North Carolina State University -- Miguel Architecture et Fonction des Macromolécules Biologiques (UMR6098) CNRS, Universités d'Aix-Marseille I & II Case 932, 163 Avenue de Luminy, 13288 Marseille cedex 9, France Tel: +33(0) 491 82 55 93 Fax: +33(0) 491 26 67 20 mailto:miguel.ortiz-lombar...@afmb.univ-mrs.fr http://www.afmb.univ-mrs.fr/Miguel-Ortiz-Lombardia
