Hi Matt, This doesn't really answer your question directly, but sidesteps around the issue - I wrote a little something on this exact subject not so long ago - http://xtaldave.wordpress.com/2012/02/23/on-protein-crystallisation/ (You can ignore the first 5 paragraphs of intro - it was written with a lay audience in mind)
Basically, I setup fine gradient screens in deep well blocks by titrating stock solutions across a row - which I can then put straight into use with our Phoenix crystallisation robot. In most situations I would hope to get decent diffracting crystals from a standard 3-well intelliplate setup this way. HTH Dave ============================ David C. Briggs PhD Father, Structural Biologist and Sceptic ============================ University of Manchester E-mail: david.c.bri...@manchester.ac.uk ============================ Webs : http://flavors.me/xtaldave Twitter: @xtaldave Skype: DocDCB ============================ On 26 March 2012 18:57, Matthew Lalonde <mattc...@gmail.com> wrote: > Dear All, > > I have searched the archives and would like more information about > reproducing robot tray hits using 24-well hand trays. I reproducibly get > crystals when I use small volumes (0.5 ul) in 3-well intelliplates but only > precipitate in 1-2 ul sitting drops in 24-well hand plates. > > What parameters should I vary to reproduce crystals in hand plates? > References that discuss this procedure exhaustively would also be > appreciated. > > Thanks, > Matt
