P21.. You sure about this space group? (very high confidences for space group and laue group in pointless?)
F On Apr 19, 2012, at 12:20 AM, LISA wrote: > Hi all, > > I am trying to solve one structure by molecular replacement with phaser in > CCP4. This a complex of a multi-domain domains with small ligand. I have > structues of this protein in apo state and with other similar ligand. The > space group of this crystal is P21. This crystal should have 4 molecules in > ASU. I used the full protein as model but did get any sol and LLG is below > zero. Then each domain were used as the search models in phaser with rotation > and tranlsation. I can the get high z score (>20), and LLG is raising. It > looks like I get the right sol, but it have more 50 clashes. Why phaser > give wrong sol with so high z socre? Can anyone give me some suggestion to > solve my strucutes? Thank you. > Best > > Lisa
