At 280nm absorbs Trp but Tyr and Phe (much less) as well. So your protein
sample even without Trp will absorb. A mass spect experiment should show a
presence of molecule with MW 507 (ATP) or MW 427 (ADP)

 

 

 

George 

 


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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Xinghua Qin
Sent: Monday, June 04, 2012 7:52 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How to know if ADP exists in the ATP-binding site of
bacterial expressed proteins

 

Deer CCP4ers,

 

How to know if ADP exists in the ATP-binding site of bacterial expressed
proteins? Check the UV spectrum at 280 nm is one way, but there is no Trp in
my protein.  Is there any other conveniet way to find out? 

Thanks in advance

 

Best wishes

 

Xinghua Qin

--
Xinghua Qin 

State Key Laboratory of Plant Physiology and biochemistry 
College of Biological Sciences
China Agricultural University
No.2, Yuan Ming Yuan West Road
Haidian District, Beijing, China 100193
Tel: +86-10-62732672
E-mail: <mailto:xhqin1...@gmail.com>  xinghua...@126.com

 

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