We routinely use BSA-free anti-5His mAb from QIAGEN, and it works just fine (at
least using media and lysates of both insect and mammalian cells):
http://www.qiagen.com/products/protein/detection/qiaexpressdetectionsystem/penta-hisantibody.aspx
Good luck,
Luca
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Luca Jovine, Ph.D.
Assistant Professor & EMBO Young Investigator
Karolinska Institutet
Department of Biosciences and Nutrition & Center for Biosciences
Hälsovägen 7, SE-141 83 Huddinge, Sweden
Voice: +46.(0)8.524-81136 FAX: +46.(0)8.6081-501
E-mail: luca.jov...@ki.se
W3: http://jovinelab.org
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________________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of D Bonsor
[dbon...@ihv.umaryland.edu]
Sent: Monday, June 25, 2012 23:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Off-topic His-Antibody
Are there any good antibodies for His-tags on the market. I have never used one
and I heard several stories that they were poor and not worth the money, over
the past few years. The only post I could find on the BB was from 2008. Have
His-Antibodies improved or are they still rubbish.
