I can second this. Whether or not you get a signal with DSF depends on your
protein, not on the type of ligand. We also use Sypro orange.
Herman
________________________________
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Bosch, Juergen
Sent: Thursday, June 28, 2012 1:42 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] off topic: protein peptide interaction using DSF
Yes
add peptide in different concentrations to your protein and run the
assay*
Yes, or Sypro Orange
*Rule of thumb 20 kDa protein use 1 mg/ml 50 kDa protein use 0.5 mg/ml
also have a peptide which should not interact with your protein as
control
Jürgen
On Jun 28, 2012, at 7:34 AM, rashmi panigrahi wrote:
Hi all,
Has anyone performed protein peptide interaction experiments
using flurophore on a real time PCR machine?
Please advice me on how the experiment is performed. Can I use
ANS?
with regards
--
rashmi
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://web.mac.com/bosch_lab/
