Hi Hsu, You can check my paper. We have done a similar analysis of several factors responsible for thermal stability, analysis done using various mesophiles and thermophiles, Crystal structure of the *Bacillus anthracis* nucleoside diphosphate kinase and its characterization reveals an enzyme adapted to perform under stress conditions. Gauri Misra et al.
Proteins: Structure, Function, and Bioinformatics Volume 76, Issue 2, <http://onlinelibrary.wiley.com/doi/10.1002/prot.v76:2/issuetoc> pages 496–506, 1 August 2009 Hope it gives you some help. Best wishes, Gauri On Sat, Jun 30, 2012 at 7:45 AM, Theresa Hsu <theresah...@live.com> wrote: > Dear all > > I have two homologues structures, from a mesophilic bacterium and a > thermophilic bacterium. Is there any software or server that can calculate > the differences that contribute to thermostability, e.g. proportion of > amino acids that form Hydrogen bonds and number of prolines? > > Thank you. >
