Hi, I have used pure malonate (above 3M) for a similar condition. Sometimes the cryoprotectant alone, without reservoir works quite well
Cheers Christian Am Dienstag 10 Juli 2012 18:28:30 schrieb m zhang: > regaentDear All, > I am sure this question was discussed before. But I am wondering if anyone > got the same experience as I do. I got a crystal out of condition with 1M > KCl, 1.4M Ammonium sulfate at pH7. I tried to use glycerol, ethylene > glycol, 25% sucrose, paraton-N oil, or ammonium sulfate itself: The > problem is that all the cryo plus original reagents in the reservoir > precipitate the salts out. And more serious problem is because of high > salt in the condition, while I am trying to loop the crystal, both the > drop and cryoprotectant drop form salt crystals (not sure it is KCl or > ammonia sulfate) significantly and very quickly, that cause my crystal > dissolved. My crystal doesn't seem to survive paraton-N oil. Does anyone > here have similiar case? any suggestion will be appreciated. Thanks,Min >
