Urea?!?!? My arm hair went up. Anyways, DLS (Dynamic Light Scattering) might help. Hard to imagine something quicker and easier, provided that someone next to you already has a DLS machine.
(sorry for the late answer) Carlos Em 26/06/2012, às 15:10, Brad Bennett escreveu: > Native PAGE (i.e. BN-PAGE), light scattering (i.e. MALLS) > > Not quick and easy but could work: AUC (i.e. a sedimentation equilibrium > experiment) > > -Brad > > On Tue, Jun 26, 2012 at 9:01 AM, Careina Edgooms <careinaedgo...@yahoo.com> > wrote: > Dear ccp4 bulletin board > > I apologise for off topic question. I wonder if anybody knows of a good > method to detect oligomerisation? > I suspect an equilibrium intermediate is forming oligomers based on > tryptophan fluorescence showing an exposed tryptophan becoming buried in a > hydrophobic region. I would like to perform some experiments to prove this. > Cross linking is not working for me and neither is SEC HPLC due to technical > issues with the urea I think. > Are there any other quick and easy techniques that any of you can think of > off the cuff? Shifts in equilibrium unfolding curves done at different > concentrations will probably show something because of the law of mass action > but this is a big job needing many replicates so I wonder if there are any > other simple methods available? > > Thanks > Careina >
