Hi, I have a crystal with a large unit cell in P21221 (a=134 b=151 c=276) and I'm wondering if I have a problem with overlaps. I have a native dataset, and am trying to get phases. I've collected a Pt soak data set on our home source with a 0.5˚ oscillation angle, but the anomalous signal drops off after about 8Å. I am wondering if this is a problem due to overlaps at higher resolution.
The Pt dataset has been integrated with XDS, and there don't seem to be too many rejects, but looking at FRAME.CBF it looks like the predicted spots are overlapping at higher resolution. You can see a zoomed-in part of FRAME.CBF here: http://imgur.com/1WShV Should I be concerned with this? The crystal mosaicity from XDS is 0.25, so fairly low. What can I do about this, should I try smaller oscillation angles? Thanks, Jason. -- Jason Busby PhD Student Laboratory of Structural Biology School of Biological Sciences University of Auckland Thomas Building 110 3a Symonds St Private Bag 92019 Auckland 1142 New Zealand ph: +64 9 3737599 ext 84155 fx: +64 9 3737414
