How about just co-transfecting with two different plasmids which have different selection markers? I've done it a lot, and it seems to work fine...
JPK On Fri, Aug 24, 2012 at 10:39 PM, Lye, Ming <ming_...@hms.harvard.edu>wrote: > Dear CCP4bb, > > We would like to co-express proteins under Se-Met conditions for de-novo > phasing of a complex. One of the proteins expresses much better with a > GST-tag compared with a his-tag. > > As its binding site is at its N-terminus, we are hoping to co-express it > as a C-terminal GST tag recombinant. So far however, we haven't found any > commercially available duet vectors with such a tag. We would truly > appreciate if anyone knows of the availability of such vectors, or has any > suggestions on similar vectors. > > Thanks so much! > Ming > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: j-kell...@northwestern.edu *******************************************
