You could do homology modeling of several homologs using http://protein.cribi.unipd.it/Homer/ . Then calculate the electrostatics of the homologs.
Better though would be actual structures. How much identity do the homologues have? If they are so close that homology modeling works, then you may be able to crystallize a few with the information available from the structure you already have. Imagine how powerfully a range of experimental structures would be to support your argument that the electrostatics are a conserved feature of the protein. James On Sep 14, 2012, at 12:37 PM, Qiang Chen wrote: > Hi all, > > I'm working on a protein structure which showed a special electrostatic > potential on its surface: positive on one end and negative on the other > end. I wonder to what extent I can say this pattern is determined by the > charged residues? If the residues are conserved, could I make a conclusion > that its homologues also have such pattern? > > Thanks! > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine > at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail.
