Hi Andreas,
The simple answer to this is that you do NOT
attempt to redefine the background. Providing the additional spots
shown in your image belong to the same lattice, mosflm will
automatically exclude pixels from adjacent spots when doing the
background plane fitting, so you need do nothing. It helps to have a
larger box size because the background plane is better defined.
To answer your question, you need to go to "Settings -> Processing
Options -> Advanced integration" in imosflm, then the top 5 lines
define the measurement box parameters, changing the box width and
height should do what you want, although you may need to uncheck the
box "Optimise overall box size" to stop the box being made bigger again.
Best wishes,
Andrew
On 17 Sep 2012, at 15:03, Andreas Förster wrote:
Hi all,
I'm integrating data from a crystal with a fairly long axis. In
iMosflm (1.0.7), the background definition is so generous that
plenty of pixels from adjacent peaks are included (see attached).
Can someone tell me how I redefine the background to be tighter
around the spots?
Thanks.
Andreas
--
Andreas Förster, Research Associate
Paul Freemont & Xiaodong Zhang Labs
Department of Biochemistry, Imperial College London
http://www.msf.bio.ic.ac.uk
<backgroundSpots.png>
