Alexander, We produce baculovirus at Gibco SF900-II + 10% heated FBS + 1xPSG and then store at 4C routinely. And it seems to be quite stable. We have a virus that had no change for more than 2 years: we judge by the volume of it needed to infect_cells/produce_protein, no titer measurements. We also avoid exposure to light: definitely cover it with foil if it's transparent door fridge.
Vitali On Thu, Sep 27, 2012 at 5:42 PM, aaleshin <aales...@burnham.org> wrote: > Sorry for an off-topic question. > > We began experiencing a sudden reduction in stability of baculovirus stock > stored at 4C, like the titer drops more than 5 fold in 3-5 month. The only > difference compared with previous preparations is a switch from Gibco > SF900-II to a media from Lonza (Insect-XPRESS). > > Could it be due to the media switch, or something else? What is the best > way to store baculovirus? > > Thank you > Alexander Aleshin > Sanford-Burnham Medical Research Institute > Infectious & Inflammatory Disease Center > 10901 North Torrey Pines Road > La Jolla, California 92037 >
