Doesn't seem like there'd have been much isopropanol left in that tube
after 2 years. In fact, your PEG may have gone off as well, changing
the pH and god knows what else. Try screening pH and leaving out
isopropanol in your optimization.
phx
On 3 October 2012 17:37, Peter Hsu <hsuu...@u.washington.edu
<mailto:hsuu...@u.washington.edu>> wrote:
Hi all,
I recently got some hits from a Mosquito grown at 18C with PEG4000
and 10% propanol. I've tried reproducing these hits using the
standard 24 well format with no success, even with playing with
PEG and propanol concentrations. The initial screening solution
was from a kit that's been sitting in the cold room for the better
part of 2 years. Does anyone here have any tips or tricks in
reproducing crystals grown from organic solvents?
Many thanks in advance,
Peter
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