Hi, Using a surface property analysis (3D structure is available), I think you can get a quantitative estimate by using PISA to find the solvent-accessible area and salvation energy and then comparing that to corresponding values obtained by using in your lab another protein for calibration (maybe ones that you label as highly, medium or minimal soluble with known mg/ml).
If you just want an estimate from sequence analysis for solubility on heterologous overexpression, you can try: SOLpro in http://scratch.proteomics.ics.uci.edu/ Or http://mips.helmholtz-muenchen.de/proso/proso.seam http://www.biotech.ou.edu/ Thanks, Debanu -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Tommi Kajander Sent: Friday, October 12, 2012 10:23 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] solubility estimates for domains/structures? Hi all, Does anyone a program/paper that would give some quantitative estimate for protein solubility based on surface property analysis? (excluding obvious things such as integral membrane / TM regions) Best Tommi
