I think it's MPD. I have also observed in my crystals. Amit Luthra, Ph.D. Post-Doctoral Fellow The Radolf Laboratory Department of Medicine University of Connecticut Health Center
[e] alut...@uchc.edu<mailto:alut...@uchc.edu> [p] 860/ 679 - 8390 ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Vandna Kukshal [vanx...@gmail.com] Sent: Wednesday, October 17, 2012 12:38 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] unidentified density I think it might be PEG. u should try to fit that. On Wed, Oct 17, 2012 at 7:13 AM, RHYS GRINTER <r.grinte...@research.gla.ac.uk<mailto:r.grinte...@research.gla.ac.uk>> wrote: Is this density on an 2 fold axis? ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>] On Behalf Of Sudhir Kumar [sudhir.1...@gmail.com<mailto:sudhir.1...@gmail.com>] Sent: 17 October 2012 12:07 To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] unidentified density Dear all, I have been working on the crystal structure of an enzyme in which I found the unidentified density (see images in attachments). Crystallization condition has Peg 1000, Peg 1500, Ethylene glycol, Tris and MPD. Does any one has any idea what it could be? Thanks -- best regards Sudhir Kumar Research Scholar C/O Dr. S. Gourinath Structural Biology Laboratory SLS, JNU, New Delhi-110067 -- Vandana kukshal