Dear all, What's the difference between "no prior phase information", "phase and FOM", Hendrickson-Lattman coefficients", and "SAD data directly" in the refmac5 GUI of CCP4i?
I have a SAD dataset and solve the phase by phenix.autosol. Now I want to refine the structure by refmac5, which kind of input above I should choose? Another question is in the "Refinement Parameters" of refmac5. What's the meaning of "Use experimental sigmas to weight Xray terms"? If I use molecular replacement to solve the structure, shall I "uncheck" this item? Thanks very much for your help. Best wishes, Q. Cai
