Hi,
to my knowledge, Ellman's reagent detects free thiols only. (Or am I
wrong?) In contrast, with the method of Thannhauser (Thannhauser [1987],
Methods Enzymol. 143, 115-9) you can determine the total amount of thiols
(free and in disulfide bonds). Any difference between the two methods
should indicate the presence of disulfide bonds, but will probably not
allow for their actual quantification.
Regards,
Joern
******************************************
Address:
Joern Krausze
Molecular Structural Biology
Helmholtz Centre for Infection Research
Inhoffenstrasse 7
38124 Braunschweig
Germany
Email: joern.krau...@helmholtz-hzi.de
Phone: +49 (0)531 6181 7023 (office)
+49 (0)531 6181 7020 (lab)
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On Wed, 6 Feb 2013, Yuri Pompeu wrote:
Dear All,
I am trying to probe the existence of a disulfide bond on the surface of my
protein.
I have attempted Ellman´s and my results were not as clear as I would have
hoped for.
I am not a sulfur/cysteine chemist and would appreciate the advice on what
experiments to try!
Thanks a bunch
YAP
