Faisal, I guess you have to update the water molecules yet. In most places it looks like density for water molecules. The density near T279 may be acetate ion. It is just a guess based on the shape of electron density and the direction of the N-H moieties..etc. (it is hard to see 3d here). I do not want to guess too much and put you in trouble. It appears that you have around 2.5A data...don't extrapolate your imagination too much and get caught by the program TWILIGHT!!!!
Ramagopal > Dear all > > I request you to please tell me the missing molecule in the electron > density map of my protein (in the attachment)..i had purified my protein > in > tris buffer however the crystallization buffer was the mixture of bicine > and trizma having pH 8.5. The precipitant was a mixture of Peg 20,000 and > peg 550 mme and for additives i had used the mixture of Sodium formate, > ammonium acetate, trisodium citrate, sodium potassium tartarate and sodium > oxamate..Please help me out i am confused.. > > -- > Regards > > Faisal > School of Life Sciences > JNU >
