Dear Pavel,

Is this density located on a symmetry axis?  
There is quite often a lot of "noise" around these regions - and it may not, in 
fact, be possible to model this satisfactorily.

Tony.


---
Dr Antony W Oliver
Senior Research Fellow
CR-UK DNA Repair Enzymes Group
Genome Damage and Stability Centre
Science Park Road
University of Sussex
Falmer, Brighton, BN1 9RQ

email: antony.oli...@sussex.ac.uk
tel (office): +44 (0)1273 678349
tel (lab): +44 (0)1273 677512

On Feb 25, 2013, at 10:03 AM, Natashin Pavel wrote:

> Hello everyone,
> 
> 
> I’m working on a structure with a 1.72Å resolution data and almost finished 
> it. But there is a piece of unknown density between two protein molecules. 
> The following pictures show this density from two sides:
> 
> http://www.4sync.com/photo/WHewU7Ek/Density_1.html
> 
> 
> http://www.4sync.com/photo/RM3IEesw/Density_-_2.html
> 
> This density is located between two protein molecules and is not connected to 
> either of them.
> 
> I have tried to fit several small organic molecules from crystallization 
> conditions and protein sample buffer, also tried ligand database search using 
> “Phenix ligand identification” software, but no satisfactory results. 
> Crystallization condition: DL-Malic acid
> 
> Sample Buffer: Bis-Tris, EDTA
> 
> Reagents from protein purification step (also checked): Tris, Urea, 
> Triton-X100, DTT. 
> 
> 
> 
> I will very much appreciate to hear any suggestions and ideas on what to do.
> 
> Best regerds, 
> 
> Pavel V. Natashin
> 
>  
> PhD student
> Photobiology Laboratory 
> Institute of Biophysics
> Russian Academy of Sciences Siberian Branch 
> Krasnoyarsk 660036, Russia 
> 
> National Laboratory of Biomacromolecules
> Institute of Biophysics Chinese Academy of Sciences
> Beijing 100101, China

Reply via email to