Dear all, currently, I have a data set scaled in P22121 which containing a PST of (0.5,0.5,0.111). The structure were successfully solved by molecular replacement. However, the R free factors remained as high as ~33% in the refinement. I search the literature and found that it was common to have such high R free factors in case of PST (Felix F.Vajdos,etc.,protein science,1997;Arthur H.Robbins,etc.,Acta D,2010;Florence Poy,etc,NSMB,2001;Cory L.Brooks etc,Acta D,2008;). In the 2001 NSMB paper( doi:10.1038/nsb720), the authors split the dataset into 'weak,medium and strong' reflections, and showed good refinement statistcs in the 'medium reflection dataset'. Although I had good electron density maps to show my solution is correct. To further convince the reviewers, I also want to split my data set into such sub-datasets according to the symmetry. Did anyone know how to split the data set in this case?
Best regards, Yuan
