Although it is not "mandatory", I think it would be a very good idea, especially if you have the exact same spacegroup and the native and ligand-bound forms of your protein are essentially the same.
If you do not use the same reflections, you are actually not reporting an independent (free) R-factor. Best regards, Folmer 2013/2/28 Prof. K. Sekar <se...@physics.iisc.ernet.in> > Dear Kavya, > > It is not so. It is not > mandatory. > > best, > > Sekar > > > > Dear users, > > > > Is it mandatory to use the same reflections for > > Rfree calculations of a ligand bound data as that > > of its native? > > > > Thank you > > With Regards > > Kavya > > > > > > -- > > This message has been scanned for viruses and > > dangerous content by MailScanner, and is > > believed to be clean. > > > > > Could you kindly confirm the safe > receipt of this mail please > > All best wishes and regards, > > Yours sincerely, > > Dr. K. Sekar, Ph.D. > Associate Professor > Bioinformatics > Indian Institute of Science > Bangalore 560 012 > INDIA > > E-mail: se...@physics.iisc.ernet.in > > Tel: 91-(0)80-22933059, 23601409, 22932469 > Fax: 91-(0)80-23600683, 23600551 > > Homepage: http://www.physics.iisc.ernet.in/~dichome/sekhome/index.html > > > -- > This message has been scanned for viruses and > dangerous content by MailScanner, and is > believed to be clean. > -- Folmer Fredslund