Dear All,
I have encountered one problem to optimization the crystallization condition manually. Actually i got the good shape and even size crystal in a screening plate. The condition are : 20% PEG 6000, 0.1M MES pH 6.0 whereas protein in 10mM Na-acetate pH 5.7 contain 50mM NaCl, 1mM EDTA &5mM BME. When I tried to optimize this on Linbro the drops are become heavily precipitate even a mints. I tried so many things to avoid this ppt such as start PEG conc from 7%, dilute protein at half conc., add more salt in a reservoir solution but no succeeds. The protein prep is same which was using to get the Hits. I would be very thankful if you people give me nice suggestions. Thanks Best Regards AFSHAN
