I THINK IT IS PEG.. On Thu, May 16, 2013 at 10:20 PM, Nicholas Keep <n.k...@mail.cryst.bbk.ac.uk > wrote:
> It is most likely to be something in your crystallisation condition, your > cryoprotectant, or the buffer you stored your protein in. > Could be a detergent carried through several steps of purification > Could for example be some part of a PEG molecule that gets ordered round a > more hydrophobic bit of your protein surface. > You need to think about everything your protein has come into contact > with, possibly even inside the cell. It is not uncommon to carry cofactors > right through a purification, less likely in something more surface bound > like this. > Best wishes > Nick > > -- > Prof Nicholas H. Keep > Executive Dean of School of Science > Professor of Biomolecular Science > Crystallography, Institute for Structural and Molecular Biology, > Department of Biological Sciences > Birkbeck, University of London, > Malet Street, > Bloomsbury > LONDON > WC1E 7HX > > email n.k...@mail.cryst.bbk.ac.uk > Telephone 020-7631-6852 (Room G54a Office) > 020-7631-6800 (Department Office) > Fax 020-7631-6803 > If you want to access me in person you have to come to the crystallography > entrance > and ring me or the department office from the internal phone by the door > -- Regards Faisal School of Life Sciences JNU
