There are many variables you can adjust, not sure how many you have tried. Here are a few that pop up first for me:
Seeding (if growth is not well controlled) Changing drop ratio or vapor diffusion set up (sitting vs hanging vs under oil) to control the kinetic process as well as the final point in the phase diagram Changing growth temperature Additive screen The above are the easy stuff without changing the well composition. If they don’t work, you can optimize the well composition. I will start with the salt concentration, then buffer type, then other components. Good luck. --------------------------------------------------------------------------------------------------------------- Yong Wang, Ph.D. Research Advisor, Discovery Chemistry Research Eli Lilly & Company Phone: 317-655-9145 Lilly Corporate Center DC 0403 Fax: 317-651-6333 Indianapolis, IN 46285 wang_y...@lilly.com CONFIDENTIALITY NOTICE: This e-mail message from Eli Lilly and Company (including all attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of ?? Sent: Tuesday, May 28, 2013 9:19 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] how to make the crystal thicker Dear professors, I get my crystal in 0.1M Tris, PH7.5, 200mM (NH4)2SO4, and 20% PEG3350, however, it is very thin. From one side, the diffraction is perfect, about 2.2A, but from the other side, diffraction is too bad, the spots look like a thread! Process cannot be done by HKL2000. As top guns of this field, could you give me some suggestion to make the crystal thicker? I will be grateful for your kind help. Best, Jiang Yan Institute of Biophysics, Chinese Academy of Sciences Beijing, Chaoyang District
