I don't really understand what your space group is? space group mC??? Eleanor On 10 Jun 2013, at 19:57, Wei Shi wrote:
> Hi all, > I was trying to solve the structure of a protein in several different > datasets using xds and phenix. I could solve the structure from one dataset > in space group P4. For another dataset, I could solve the structure using the > monomer of the structure I got from the first dataset as search model and > solve the structure in space group mC. For the third dataset, in IDXREF.LP, > the space group of the highest symmetry is hp: 101.2, 101.3, 58.8, 90, 90, > 120. According to Mathiews coefficient, 1 monomer is expected in the > asymmetric unit. But I couldn't get the molecular replacement solution using > the same method as for the second dataset. I also tried several other search > models (eg. deletion of the potential flexible region in the search model) > and tried to find the solution in all possible pointgroup. I also tried to > process the data in oC (C222), the space group of the second highest symmetry > in IDXREF.LP, but, still I could not get right molecular replacement > solution. I don't whether this means that there is a big conformational > change for the structure in the third dataset or the space group I use is not > right. Let me know if any of you would have any comments or suggestions for > me. Thank you so much! > > Best, > Wei > >
