Hello, Monika, 1) Add NAGs 2) Remove their hydrogens and O1 atom and put them in density 3) In menu: Calculate->merge molecules, choose your pdb and both NAG residues 4) save coordinate file
2013/6/20 Monika Pathak <m.pat...@nottingham.ac.uk> > Hi > > Please can I ask about how can I put NAG to asparagine in coot (I think > its 2NAG that I can put in density) and if possible to refine it in > refmac then. > > Thanks in advance for help > Regards > Monika > > Monika Pathak > University of Nottingham > NG7 2RD > > > This message and any attachment are intended solely for the addressee and > may contain confidential information. If you have received this message in > error, please send it back to me, and immediately delete it. Please do > not use, copy or disclose the information contained in this message or in > any attachment. Any views or opinions expressed by the author of this > email do not necessarily reflect the views of the University of Nottingham. > > This message has been checked for viruses but the contents of an > attachment may still contain software viruses which could damage your > computer system, you are advised to perform your own checks. Email > communications with the University of Nottingham may be monitored as > permitted by UK legislation. > > -- Eugene Osipov Junior Research Scientist Laboratory of Enzyme Engineering A.N. Bach Institute of Biochemistry Russian Academy of Sciences Leninsky pr. 33, 119071 Moscow, Russia e-mail: e.m.osi...@gmail.com