We used expression of full length beta-galatosidase in yeast for a lab class. Simple to purify, simple to assay.
On Mon, Aug 5, 2013 at 6:55 AM, Alan F Scott <alan.sc...@bristol.ac.uk>wrote: > Dear All, > > Sorry for the off-topic post. I am looking to overproduce a protein for > crystallography purposes in *Saccharomyces cerevisiae*. Nobody in my lab > has ever overproduced a protein in yeast before so I need to try producing > a control protein first. Does anybody know an *E. coli *or yeast protein > which can be easily produced in *Saccharomyces *in large quantities > please? Thanks for any suggestions. > > Best wishes, > > Alan > -- David M. Mueller Biochemistry and Molecular Biology The Chicago Medical School Rosalind Franklin University of Medicine and Science 3333 Green Bay Road North Chicago, IL 60064 *david.muel...@rosalindfranklin.edu http://lmb-il.org/index.html *Office: 847-578-8606 FAX: 847-578-3240
