Deal Xiang, You can think of GFP/YFP protein attached with any protein would be good for your experiment. These can be easily cloned and expressed. Alternatively one more approach is mentioned below. Chose a protein which has only one cysteine, that too at the surface then you can think of labelling your protein with thiol-ester of commerically available atto-dyes. You can also think of mutating alanine present at the surface with cysteine which subsequently can be covalently attached with the thio-reactinve dyes. It is two step process, first you have to set up a chemical reaction linking the thiol mediated covalent ligation of dyes to your protein, then further purifying the lablled protein with HPLC.
On 17 September 2013 00:54, 李翔 <lixiang1...@gmail.com> wrote: > Hi everyone, > > I want to get some fluorescence labeled protein for my experiment. I wish > it is under 40K Da and have isoelectric point of 7 or slightly lower. Do > you have any suggestions on the commercially available protein please? > > Thanks very much for your help! > > Sincerely, > Xiang > > -- > Li Xiang > Department of chemistry, > Purdue University > Email：lixiang1...@gmail.com >
