Izit is an aqueous solution of methylene blue, which you can prepare simply and 
cheaply. Look here: 

http://www.ysbl.york.ac.uk/ccp4bb/2000/msg00387.html

One word of warning: not every crystal stains. I found poking crystal with hair 
or glass fibre to see if it cracks or breaks is more reliable. Salt crystals 
react to attacks from glass fibres like rocks (as indeed what they are).


From: Swastik Phulera 
Sent: Tuesday, October 15, 2013 3:59 AM
To: CCP4BB@JISCMAIL.AC.UK 
Subject: [ccp4bb] Staining Crystals with comassie

Dear All,

I am looking for a method to quickly differentiate between salt and protein 
crystals. I have been told  thats its a popular alternative to the commercially 
available izit dye. I would appreciate if some one would share their comassie 
crystal staining protocol.


Swastik

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