Can you exclude a sequencing error for Gly104? Am 31.10.2013 um 08:40 schrieb thuy ngo:
> Dear all, > > I am dealing with two strange extra density map. One is connected from Cb of > Proline to Ca of glycine (blob 1) > and other is positioned between two symmetric molecules which is connected > from Cb of Histidine to carboxylate group of Glutamate. I checked all the > crystallization and purification condition but no ligand is suitable for > these blobs. Could you suggest some possible candidates? > This is the structure complex between peptide deformylase and fMAS > Crystallization condition: 2M Sodium acetate, 0.1 M HEPES 7.5 and 0.05M CdSO4 > > Thank you > <blob1_back.png><blob1_front.png><blob2.png> Prof. Dr. Arne Skerra Lehrstuhl f. Biologische Chemie | Technische Universitaet Muenchen Emil-Erlenmeyer-Forum 5 | 85350 Freising-Weihenstephan | Germany Phone: +49 (0)8161 71-4351 | Fax: -4352 eMail: [email protected] | http://www.wzw.tum.de/bc
