Thanks Francis, No, only one molecule in the asu. The Matthews Coefficient is 3.3, corresponding solvent content is 62.6%, maybe that's why this crystal show such weak diffraction?
Zhihong On Thu, Nov 7, 2013 at 5:37 PM, Francis Reyes <francis.re...@colorado.edu>wrote: > > Do you expect more than one molecule in the asymmetric unit? > > Determined from the Matthews Coefficient (poor), size exclusion column > (better), or self RF (best) ? > > > On Nov 7, 2013, at 8:36 AM, Zhihong Yu <nkyuz...@gmail.com> wrote: > > > Hi, all > > > > I'm a rookie in resolving a brand new structure. I have some questions > for my current case and look forward to some suggestions. > > > > Now I’m working on a protein like this: > N-ter(55aa)—domainA(110aa)—linker(30aa)—domainB(150aa)—C-ter(20aa), I got a > diffraction data just to 3.5Å, and there is no complete homology structure > in pdb bank, but only a homology structure (named as structureX later) for > domainB with ~30% sequence identity, so I have some questions as following: > > > > 1. Is it possible to find a resolution through MR approach using > structureX as a search model? Especially considering that the resolution is > only 3.5Å. Currently I just tried once using phaser and refine the > structure, I can get a R/Rfree of 0.45/0.55, and it looks like most of > backbone in the structureX, especially those within helix or sheet, can be > well described by 2Fo-Fc density. Is this primary result promising or not? > > > > 2. If it’s possible, what’s the general optimal procedure I should > follow? > > > > Really thanks for any advice and suggestions! > > > > Zhihong > > > > --------------------------------------------- > Francis E. Reyes PhD > 215 UCB > University of Colorado at Boulder > > > > > > >
