''Signals in the region from 250-270 nm are attributable to phenylalanine residues, signals from 270-290 nm are attributable to tyrosine, and those from 280-300 nm are attributable to tryptophan. Disulfide bonds give rise to broad weak signals throughout the near-UV spectrum. ''
----------------http://www.ap-lab.com/circular_dichroism.htm ________________________________ From: JinSoo.Bae <jiroz...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, 15 January 2014 9:41 AM Subject: [ccp4bb] Circular Dichoism 280nm range signal Dear all, Sorry for the off topic. We are comparing the high order structure between Fc engineered mAbs using CD methods. There are some different (noise ?) signal in the 280nm range each antibodies. Other region is almost close match with respect of peak shape and theta value. Is there any specific reason why the wavelength 280nm area have a little different signal ? That will be really thanks if someone explain the reason or give a some references papers. Thanks in advance. Jin Soo Bae 790-784 room 204, Dept of Life Science, POSTECH, san31, Hyoja-dong, Nam-gu, Pohang, Gyungbuk, Korea tel:82-54-279-8627 fax:82-54-279-8111
