Thanks for all the replies. And I would not call this laziness, rather crowdsourcing....
I should have added a few more details; it is easy to export from say Unicorn to excel, a list of pairs of values, Abs280 vs elution times, with which one can recreate the chromatogram. I wanted to use these for my calculations. K On Wed, Jan 15, 2014 at 10:49 AM, Evgeny Osipov <e.m.osi...@gmail.com>wrote: > I am not sure, but probably path length not 1 cm in a detector cuvette . > You must refer to your HPLS system manual for this value > 15.01.2014 19:09, Karel Chaz пишет: > > Dear all, >> >> A question for the biochemistry-inclined folks in the bb; how do I >> calculate protein concentration of chromatography fractions, starting from >> Abs280 from the UV monitor? I know I could figure it out myself if I really >> tried, but why bother when I have access to so many brilliant minds.... >> >> >> Thanks to all, >> >> K >> > > > -- > Eugene Osipov > Junior Research Scientist > Laboratory of Enzyme Engineering > A.N. Bach Institute of Biochemistry > Russian Academy of Sciences > Leninsky pr. 33, 119071 Moscow, Russia > e-mail: e.m.osi...@gmail.com >
