Dear ccp4bb contributors,
We are dealing with the problem of a protein (~ 50 kDa) that crystallises
readily, but has an annoying habit of forming highly intergrown rods or
needles.
Even when the crystals look optically homogenous under the microcsope,
diffraction is so so (3.5 Å or so on the synchrotron), but patterns reflect
several crystal lattices that the processing software cannot resolve properly.
We have tried this:
- additive screens
- switching the His-tag from N- to C-terminus
- cutting the tag
- thermal stability screens in a variety of buffers
- growth in the presence of potential ligands/substrates
Any suggestions for tricks that we haven't thought of so far?
Thank you.
Klaus
=======================================================================
Dr. Klaus Fütterer
School of Biosciences P: +44-(0)-121-414 5895
University of Birmingham F: +44-(0)-121-414 5925
Edgbaston E: k.futte...@bham.ac.uk
Birmingham, B15 2TT, UK W: http://tinyurl.com/futterer-lab
=======================================================================
