Dear All,
Since 80% saturated Li2SO4 has not been mentioned, I will do so. It is a
good cryosalt and I have often used
it even without any buffer added.
see:
Vera, L., Stura, E. A. (2013) Strategies for protein cryocrystallography.
Crystal Growth & Design, e-print
http://pubs.acs.org/doi/full/10.1021/cg301531f
The problem with phosphate precipitants is the formation of salt crystals
in the cryoprotectant solution.
Cryoprotecting molecules that normally work well with high ionic strength
conditions do not always
work well with phosphates.
800 mM Sodium phosphate monobasic/1200 mM Potassium phosphate dibasic
is well matched by 80% saturated lithum sulphate in terms of ionic
strength.
Enrico.
On Fri, 07 Feb 2014 06:20:58 +0100, Tanner, John J.
<tanne...@missouri.edu> wrote:
Try L-proline. It works well with high ionic strength conditions:
http://www.ncbi.nlm.nih.gov/pubmed/22868767
Sent from Jack's iPad
On Feb 6, 2014, at 10:40 PM, "Deepak Thankappan Nair"
<deepaktn...@gmail.com<mailto:deepaktn...@gmail.com>> wrote:
Hello,
Does anybody know what would be a good cryoprotectant for the following
condition:
800 mM Sodium phosphate monobasic/1200 mM Potassium phosphate dibasic
100 mM Sodium acetate/Aceticacid pH4.5
Thanks
Deepak
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
