Hi Raji,
There are also some proprietary stains such as the "660 nm" (can't
they think of a better product name?) stain from Pierce that are detergent
compatible. I used this briefly with success when comparing against Abs 280
nm.
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
h...@hawaii.edu
Date: Thu, 13 Feb 2014 10:06:12 -0500
> From: Raji Edayathumangalam <r...@brandeis.edu>
> Subject: Determining concentration of membrane protein
>
> Dear CC4BBers,
>
> I am trying to figure out what is the best way to determine the protein
> concentration of my membrane protein. My purified membrane protein is in
> 20mM Tris pH 7, 150mM NaCl and 0.02% DDM (CMC of DDM=0.0076%).
>
> After reading the friendly manuals and searching online, I've learned that
> detergents interferes with assays like Bradford but can't find good
> descriptions of what works best. For now, I am trying to estimate
> concentration from absorbance at 280nm and using molar extinction
> coefficients based on aromatic amino acids, but again suspect detergent
> interference. I would like to know what other folks working on membrane
> proteins are doing.
>
> Thanks very much.
> Raji
>
